CPT Publications – Xenon Cell Preservation Research

Kelly K, Sen S, Ilyin I, Dumont LJ. Hyperbaric treatment of platelets extends in vitro storage to 14 days. Transfusion. 2022 Sep;62(9):1736-1742. doi: 10.1111/trf.17048. Epub 2022 Aug 2. PMID: 35919959

Abstract

Background: Platelets for transfusion have a storage time of 5-7 days at 22°C-24°C, which results in a strain on the supply chain and supply shortages. We describe a novel method to extend platelet storage using xenon (Xe) gas under high pressure and refrigeration.

Study design and methods: Apheresis platelets (APU) prepared in 65% platelet additive solution (PAS) were stored under standard conditions (SC) at 20°C-24°C to Day 5. Paired APUs were prepared with Xe and stored to Day 14 at 2°C-6°C under hyperbaric conditions (XHC). A standard panel of in vitro assays was conducted.

Results: XHC platelets were viable out to Day 14. The average pH of Day 14 platelets was 6.58, and 86% maintained some degree of swirl compared with 7.02 and 100% swirl for Day 5 SC platelets. The rate of glycolysis was reduced under XHC storage with less glucose consumption and lactate generation. Activation levels for Day 14 platelets, while increased, did not prevent response to agonists in vitro, including epinephrine + Adenosine 5-Diphosphate (EPI/ADP) and thrombin receptor-activating peptide (TRAP) aggregation. Thromboelastogram (TEG) assessment showed 80% or greater conservation of platelet function for Day 14 xenon stored platelets compared with Day 5 SC platelets.

Discussion: Platelet storage with the Xe/hyperbaric/cold method is a feasible candidate for extension of storage to 14 days based on in vitro characteristics. In vivo recovery and survival studies are indicated. The capability to extend platelet storage to 14 days would make large strides toward resolving issues of platelet outdating for prophylactic use.

Keywords: cold storage; extended shelf life; hyperbaric; platelet storage; xenon.

Functionality of Chilled Platelets Stored under Hyperbaric Xenon

AABB Annual Meeting & CTTXPO, Denver, CO, October 12-15, 2013

Andrew P. Cap, Kristin Reddoch, Robbie Montgomery, Heather F. Pidcoke, Anand Ramasubramanian, Alexander Shumeev, Yana Filkina, Ilya Ilyin, Louise H. Herschel, Susan L. Waters, Larry J. Dumont

Storage of Refrigerated Platelets Under Hyperbaric Pressure of Xenon.

AABB Annual Meeting & TXPO, Boston, MA, October 6-9, 2012

Ilyin I., Shumeev A., Filkina Y., Enukashvily N., Ilyina N., Kogan S.

A New Storage Method For Platelet Plasma.

AABB Annual Meeting & CTTXPO, San Diego, CA, October 22-25, 2011

Ilyin I., Shumeev A., Enukashvily N., Filkina Y., Owen W., Kogan S.

RBC Storage with Xenon Gas Protects Against Hemolysis.

AABB Annual Meeting & CTTXPO, September 2014

Dumont L.J., Herschel L., Waters S.L., Ilyin I.

The CPT Publications section highlights key scientific studies supporting the development of xenon-based cellular preservation technologies. These publications demonstrate the potential of xenon gas to significantly improve the storage, stability, and functionality of biological cells, particularly platelets and red blood cells.

A landmark peer-reviewed study published in Transfusion (2022) demonstrated that platelets stored under hyperbaric xenon conditions at low temperatures remain viable for up to 14 days. This represents a major advancement compared to the current standard storage period of 5–7 days. The study showed preserved platelet function, reduced metabolic activity, and maintained responsiveness to activation signals.

Additional research presented at AABB Annual Meetings further validates the effectiveness of xenon in preserving platelet integrity and reducing hemolysis in red blood cells. These findings confirm that xenon-based storage methods can protect cellular structures, reduce degradation, and maintain therapeutic performance over extended periods.

The collected body of research supports the use of xenon gas in combination with controlled temperature and pressure as a powerful tool for next-generation cell preservation. These advancements have the potential to transform blood banking, improve transfusion outcomes, and address global supply challenges.

General Biophysics continues to build on this scientific foundation, translating validated research into practical technologies for clinical and industrial applications.

What are CPT publications?

CPT publications are scientific studies and conference papers focused on xenon-based cellular preservation, including platelet storage and blood component stability.

What is the key breakthrough in CPT research?

Research shows that xenon under hyperbaric conditions can extend platelet storage from 5 days to up to 14 days while maintaining functionality.

Are these studies peer-reviewed?

Yes, some publications are peer-reviewed, including studies published in journals such as Transfusion, along with presentations at major scientific conferences.

What does xenon do in platelet storage?

Xenon helps stabilize cellular structures, reduce metabolic activity, and protect platelets from degradation during extended storage.

Why is extended platelet storage important?

Longer storage improves supply chain efficiency, reduces shortages, and enhances the availability of life-saving blood components.